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Image Search Results
Journal: Scientific Reports
Article Title: MSR1 is not required for obesity-associated inflammation and insulin resistance in mice
doi: 10.1038/s41598-023-29736-0
Figure Lengend Snippet: Male Msr1KO mice have decreased fat mass and inflammation compared to non-littermate wild-type controls. ( a ) Whole body weight for 12-week HFD fed Msr1KO (red) and C57BL6 wild type non-littermate controls (white). ( b ) Organ weight for Msr1KO (red) and WT non-littermate controls (black). ( c ) Insulin tolerance test (ITT) after 12 weeks of HFD. Flow cytometry analysis of ( d ) CD45 + leukocytes, ( e ) CD64 + ATMs, ( f ) CD64 − CD11c + ATDCs, ( g ) CD11c + ATMs, ( h ) CD11c + ATMs, and ( i ) CD64 + Ki67 + ATMs in SVF after 12 weeks of HFD. n = 5/group. * p < 0.05. Statistical Analysis = T-test. Identical results observed when data was expressed as percent total SVF cells.
Article Snippet: The following conjugated antibodies were used: CD301 APC (Clone ER-MP23, BioRad, Cat# MCA2392A647), CD45 Pacific Blue (Clone 30-F11, eBiosciences, Cat# 48,045,182),
Techniques: Flow Cytometry
Journal: Nature Communications
Article Title: The circadian clock influences T cell responses to vaccination by regulating dendritic cell antigen processing
doi: 10.1038/s41467-022-34897-z
Figure Lengend Snippet: a A schematic summarising how ZT time can be inferred from the in vitro synchonised serum shock model comparing Per2 mRNA oscillation in BMDCs. Shading represents the relative active periods. As mice are nocturnal, 12 h post synchronisation represents ZT0 the onset of the inactive phase, whereas 24 h post synchronisation represents ZT12 the onset of the active phase. b Per2::luciferase BMDCs were synchronised by serum shock and circadian rhythms were measured using lumicycle technology ( n = 3 biologically independent samples). Bmal1 +/+ and Bmal1 − / − BMDCs were synchronised and antigen processing was measured at c 4 h or d 12 h intervals over a 48 h time course ( n = 3 independent experiments). Antigen processing was measured by addition of DQ-OVA (1 µg/mL) and fluorescence (blue – DAPI, green – DQ-OVA) was measured at 15 min (uptake) or 60 min (processing) and then fixed and analysed by confocal microscopy. e Spleens were isolated from WT mice at ZT1, ZT7, ZT13 and ZT19 and single cell suspension generated, stained for DQ-OVA (1 μg/mL) as in ( c , d ) and subsequently stained for CD11b+ and CD11c+ and analysed by flow cytometry ( n = 4 mice). f Spleens isolated from Bmal1 myeloid+/+ and Bmal1 myeloid − / − mice and stained for DQ-OVA as in ( c , d ) and CD11b + and analysed by flow cytometry ( n = 3 mice) p = 0.0045. g , h Splenic DCs were expanded by B16-FLT3L cells. g cDCs, cDC1s, cDC2s, plasmacytoid DCs and macrophages, or h migratory and resident DCs were identified by flow cytometry and DQ-OVA processing quantified by flow cytometry ( n = 3–4 mice). cDC p = 0.003, cDC1 p = 0.005, cDC2 p = 0.04, pDC p = 0.02, macs p = 0.001, migratory DCs p = 0.01, resident DCs p = 0.0092 Data shown is mean with error bars representing ± SEM. Luciferase data was analysed for circadian rhythmicity by JTK cycle ( b ). Antigen processing in Bmal1 +/+ was predicted to be circadian by cosinor analysis ( c ). Data were compared by one-way ANOVA with Tukey’s post-hoc test for multiple comparisons ( e ) or by a two-tailed t -test ( f–h ). * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001. Source data are provided as a Source Data file.
Article Snippet: Antibodies used as follows: CD3-APC (145-2c11, Biolegend), F4/80-AF700 (cat. MCA497A700, BioRad), LY6G/6C-APC-Cy7 (RB6-8C5, BD), NK1.1-BV421 (PK136, Biolegend), MHCII-BV711 (M5/114, BD), CD11c-BV785 (N418, Biolegend),
Techniques: In Vitro, Luciferase, Fluorescence, Confocal Microscopy, Isolation, Generated, Staining, Flow Cytometry, Two Tailed Test